The potential use of plasma and serum for molecular diagnosis has generated interest. Tumour DNA has been found in the plasma and serum of cancer patients, and molecular analysis has been done on this material. We difference between serum and plasma pdf the equivalent condition in pregnancy—that is, whether fetal DNA is present in maternal plasma and serum.

We used a rapid-boiling method to extract DNA from plasma and serum. DNA from plasma, serum, and nucleated blood cells from 43 pregnant women underwent a sensitive Y-PCR assay to detect circulating male fetal DNA from women bearing male fetuses. 30 maternal serum samples, from women bearing male fetuses. These results were obtained with only 10 μL of the samples. 30 samples gave a positive Y signal. None of the 13 women bearing female fetuses, and none of the ten non-pregnant control women, had positive results for plasma, serum or nucleated blood cells.

Our finding of circulating fetal DNA in maternal plasma may have implications for non-invasive prenatal diagnosis, and for improving our understanding of the fetomaternal relationship. Check if you have access through your login credentials or your institution. What are Corrected Proof articles? 68 55 55 55 14.

18 45 45 0 12. We developed a sensitive sandwich enzyme-linked immunosorbent assay to measure GPIHBP1 in human plasma or serum. A slight trend toward higher serum GPIHBP1 levels was observed in a cohort of patients with coronary heart disease, but this finding needs to be tested in larger patient cohorts. GPIHBP1 levels in human blood. GPIHBP1 levels tended to be slightly higher in patients who had a major cardiovascular event after revascularization.

We developed an ELISA for quantifying GPIHBP1 in human blood. The potential of plasma GPIHBP1 as a biomarker for metabolic or cardiovascular disease is yet questionable but needs additional testing. The potential use of plasma and serum for molecular diagnosis has generated interest. Tumour DNA has been found in the plasma and serum of cancer patients, and molecular analysis has been done on this material. We investigated the equivalent condition in pregnancy—that is, whether fetal DNA is present in maternal plasma and serum. We used a rapid-boiling method to extract DNA from plasma and serum.